{"created":"2023-06-19T11:10:46.628458+00:00","id":925,"links":{},"metadata":{"_buckets":{"deposit":"3882bf41-828e-454e-a71d-48ba9473dc07"},"_deposit":{"created_by":5,"id":"925","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"925"},"status":"published"},"_oai":{"id":"oai:fukuoka-edu.repo.nii.ac.jp:00000925","sets":["71:74:1715571888893"]},"author_link":["2746","2644","6407"],"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-02-10","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"59","bibliographicPageStart":"47","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"福岡教育大学紀要. 第三分冊, 数学・理科・技術科編"},{"bibliographic_title":"Bulletin of Fukuoka University of Education. Part III, Mathematics, natural sciences and technology"}]}]},"item_16_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ステロール生合成遺伝子が，生物進化の過程で如何にして創生されてきたかを探究するため，単細胞の真\n核生物には見出されないが，より高等な多細胞真核生物には見出されるDHCR7遺伝子の構造を複数の生物\nで比較する目的で，まずメダカO7yzjasZatipesのDHCR7の遺伝子構造の解明に取り組んだ。ヒトHbmo\nsqpie7zsやゼブラフィッシュDa7zZorerjoのDHCR7のcDNA構造でＮＢＲＰメダカゲノムデータベースを\n対象にBLAST検索を行った。その結果，メダカO7yZjasZatjPesのDHCR7の第２と第３exonの候補\nを見出した。メダカDHCR7と考えられる塩基配列からプライマーを設計し，野生メダカのゲノムを鋳型と\nしてＰＣＲを行ったところ，推定第２exonと似た長さの産物が増幅し，メダカOZyZiaSZatiiPeSDHUR7第\n２exonの存在を確認することができた。しかし，intronと考えられる塩基配列から設計したプライマーで\nは産物の増幅が見出されなかった。メダカゲノムデータベースに採用されたメダカ近交系Hd-rR系と同じ\n種である野生メダカとで比較すると，ｅｘｏｎの塩基配列は保存されている一方，intronに種内変異が蓄積し\nている可能性が考えられた。                   \n\\n------------------------------------------------------------------------------------------\n                                                   \n\\nThe gene structure of Medaka Oryzias latipes DHCR7 was investigated through BLAST search\nby comparison of Medaka genome database with the cDNA and exon base sequences of Homo\nsapiens and Danio rerio in order to elucidate the mechanism of biological evolution at the viewpoint\nof generation of sterol biosynthesis genes. As the results, the exons 2 and 3 of DHCR7 of Medaka\nOryzias latipes were extracted as the strong candidates. One of the estimated exons, exon 2 of\nMedaka DHCR7 was amplified by using wild type Medaka genome DNA as the template, but not\nthe intron between exons 2 and 3. These results indicate the intra-species mutation in the intron\nbase sequence of Medaka Oryzias latipes.","subitem_description_type":"Abstract"}]},"item_16_radio_19":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"紀要論文 Article"}]},"item_1714138083653":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0532-811X","subitem_source_identifier_type":"PISSN"}]},"item_1714138754612":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"福岡教育大学"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"西山, 智子","creatorNameLang":"ja"},{"creatorName":"ニシヤマ, サトコ","creatorNameLang":"ja-Kana"},{"creatorName":"NISHIYAMA, Satoko","creatorNameLang":"en"}],"familyNames":[{"familyName":"西山","familyNameLang":"ja"},{"familyName":"ニシヤマ","familyNameLang":"ja-Kana"},{"familyName":"NISHIYAMA","familyNameLang":"en"}],"givenNames":[{"givenName":"智子","givenNameLang":"ja"},{"givenName":"サトコ","givenNameLang":"ja-Kana"},{"givenName":"Satoko","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2746","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"清水, 誠之","creatorNameLang":"ja"},{"creatorName":"シミズ, ノブユキ","creatorNameLang":"ja-Kana"},{"creatorName":"SHIMIZU, Nobuyuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"清水","familyNameLang":"ja"},{"familyName":"シミズ","familyNameLang":"ja-Kana"},{"familyName":"SHIMIZU","familyNameLang":"en"}],"givenNames":[{"givenName":"誠之","givenNameLang":"ja"},{"givenName":"ノブユキ","givenNameLang":"ja-Kana"},{"givenName":"Nobuyuki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2644","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"西野, 秀昭","creatorNameLang":"ja"},{"creatorName":"ニシノ, ヒデアキ","creatorNameLang":"ja-Kana"},{"creatorName":"NISHINO, Hideaki","creatorNameLang":"en"}],"familyNames":[{"familyName":"西野","familyNameLang":"ja"},{"familyName":"ニシノ","familyNameLang":"ja-Kana"},{"familyName":"NISHINO","familyNameLang":"en"}],"givenNames":[{"givenName":"秀昭","givenNameLang":"ja"},{"givenName":"ヒデアキ","givenNameLang":"ja-Kana"},{"givenName":"Hideaki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6407","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-09-28"}],"displaytype":"detail","filename":"3006-nishiyama-hoka-2012.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"3006-nishiyama-hoka-2012.pdf","url":"https://fukuoka-edu.repo.nii.ac.jp/record/925/files/3006-nishiyama-hoka-2012.pdf"},"version_id":"f0bbdbc1-4a57-40cd-abe7-390de237e5a3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ETYP:教育関連論文","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"メダカケノム情報によるステロール生合成遣伝子の解析 ～メダカDHCR7の遣伝子構造～","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"メダカケノム情報によるステロール生合成遣伝子の解析 ～メダカDHCR7の遣伝子構造～","subitem_title_language":"ja"},{"subitem_title":"メダカ ケノム ジョウホウ ニヨル ステロール セイゴウセイ イデンシ ノ カイセキ メダカ DHCR7 ノ イデンシ コウゾウ","subitem_title_language":"ja-Kana"},{"subitem_title":"Analysis of sterol biosynthesis genes from the genome information of Medaka Oryzias latipes The estimation of gene structure of Medaka DHCR7","subitem_title_language":"en"}]},"item_type_id":"16","owner":"5","path":["1715571888893"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-04-04"},"publish_date":"2012-04-04","publish_status":"0","recid":"925","relation_version_is_last":true,"title":["メダカケノム情報によるステロール生合成遣伝子の解析 ～メダカDHCR7の遣伝子構造～"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2024-05-23T05:19:19.355924+00:00"}