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遺伝子水平伝播による原核生物の形質転換に関わる因子の解析と考察
http://hdl.handle.net/10780/891
http://hdl.handle.net/10780/891f717ff7b-ab66-4b56-9bf7-73d8e2506cc7
名前 / ファイル | ライセンス | アクション |
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3005-etoh-nishino_2010.pdf (1.3 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2010-03-15 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 遺伝子水平伝播による原核生物の形質転換に関わる因子の解析と考察 | |||||
タイトル | ||||||
言語 | ja-Kana | |||||
タイトル | イデンシ スイヘイ デンパ ニヨル ゲンカク セイブ ツノ ケイシツ テンカン ニ カカワル インシ ノカイセキ ト コウサツ | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Analysis of natueal factors in transformation mechanism of prokaryotic cells by the horizontal gene transfer | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ETYP:教育関連論文 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
江藤, 夕貴
× 江藤, 夕貴× 前田, 紗綾香× 西野, 秀昭 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | For the purpose of basic research for examination of the mechanism of biological evolution through the horizontal gene transfer, it was experimented whether Escherichia coli, one of prokaryotes in this era, could be transformed by the foreign DNA under the natural condition rather than the experimental condition used for efficient transformation techniques or not. As the results, the following conclusions were obtained for the horizontal gene transfer in prokaryotes: the transformation could happen by foreign DNA even without heat shock condition (transformation efficiency, 0.05%); the concentration of calcium ion needed for the natural transformation was very comparable (10mM) to the content in earth surface soil. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 遺伝子の水平伝播による生物進化の機構を探究するための基礎研究を目的として、原核生物である大腸菌を用いて、実験研究で行われている人工的な条件による効率的な形質転換ではなく、より自然環境に近い条件下で形質転換が起こりうるかを検討した。その結果、それまで知られていた実験条件で必須とされているヒートショックが無くても(形質転換効率0.05%)形質転換は起こった。最低限、形質転換に必要なカルシウムイオン濃度は10mM(形質転換効率0.002%; 通常の100mMで0.1%ほど)で、地球表層の土壌中に含まれる平均的な濃度とほぼ同様かそれ以下であり、外来DNAによる原核生物の形質転換は自然な環境条件で充分に起こりうることであることが明らかになった。 | |||||
書誌情報 |
福岡教育大学紀要. 第三分冊, 数学・理科・技術科編 Bulletin of Fukuoka University of Education. Part III, Mathematics, natural sciences and technology 巻 59, p. 45-51, 発行日 2010-02-10 |
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出版者 | ||||||
出版者 | 福岡教育大学 | |||||
資源タイプ | ||||||
値 | 紀要論文 Article |